Transcriptome sequencing, or RNA sequencing, offers a detailed picture of cell function and behavior by looking at RNA transcription. Our RNA sequencing service provides a range of possibilities to study total RNA, mRNA or other RNA fractions with high precision and reliability.
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A transcriptome is a collection of all RNA transcripts present in a sample. RNA molecules are based on the sequence of the DNA from which they were transcribed, but this process is far from straightforward. Analyzing the transcriptome with RNA seq enables researchers to determine whether specific genes are expressed, at which rate, and what their exact sequences look like.
Similar to whole genome sequencing, there is a distinction between transcriptome resequencing and de novo transcriptome sequencing. During resequencing, fragments of complementary DNA (cDNA) are aligned onto a reference genome, whereas with de novo sequencing cDNA fragments are assembled without using a reference.
The main segments of the transcriptome for research include messenger RNA (mRNA), ribosomal RNA, transfer RNA, small RNA, extracellular RNA and long noncoding RNA (lncRNA). These can be analyzed individually or in combinations, including:
Whole transcriptome sequencing (WTS), or total RNA sequencing, gives you the most comprehensive insights into gene transcription. Our WTS service gives you information on mRNA as well as other RNA subpopulations, such as lncRNA, that can play a key role in cell behavior and function.
mRNA sequencing studies the fraction of RNA that is translated into proteins and is one of most popular methods for analyzing disease states and biological processes. This method is extremely in-depth, enabling researchers to find variants that are often undetectable with other techniques.
Preparation of mRNA libraries works by separating mRNA from the other RNA fractions. Each mRNA molecule has a series of ‘A’ nucleotides at the 5’ end (poly A tail), which can be used for selective capture.
Small RNA sequencing targets and isolates small RNA molecules to discover how these noncoding RNAs affect gene expression. Small RNAs are known to be involved in regulation of gene expression in cellular process.
Our NGS workflow targets RNAs of up to 200 nucleotides long, so it captures all small RNA families in a sample, including microRNA, small interfering RNA (siRNA), piwi-interacting RNAs (piRNA) and other non-coding RNAs.
Extracellular RNA, or exosome RNA, is RNA contained in exosomes that exist outside a cell in fluids such as plasma or urine. Isolating exosomes enables you to study the RNA contained inside them to gain deeper insight into the role of exosomes in biological processes.
By creating a full-length transcript of an RNA molecule, you can characterize the transcript isoform across the entire target gene. This technique provides accurate information about alternately spliced exons and transcription start sites (TSSs) that is difficult to obtain with short read sequencing.
1. Depending on your request, download the Custom Peptide Synthesis form or Modification Peptide Synthesis form.
2. Fill in the customer information fields
3. Upload the filled Peptide Synthesis form and Submit
4. Our colleagues will contact you
1. Depending on your request, download the Single Strand RNA Synthesis form or Double Strand RNA Synthesis form.
2. Fill in the customer information fields
3. Upload the filled Peptide Synthesis form and Submit
4. Our colleagues will contact you
1. Download the Gene Synthesis form and fill it with the necessary information. You can add more than one gene.
2. Fill in the customer information
3. Upload the filled Gene Synthesis form and Submit
4. Our colleagues will contact you as soon as possible
There are multiple types of transcriptome analysis that need specific library kits. We got you cover. Our partnership with to leader-suppliers, such as Illumina and PacBio, allows us to deal with any type of sample. Have a look at our top-selling kits.
You can have a look to the list of all library kits available and the Macrogen Europe sample criteria. RTR samples? You can also find the library criteria in case your sample are ready for sequencing.
Now, if you like what you read or you have any extra questions, you can book a free online meeting to discuss your project with our consultants. Tell us about your project and we will get the correct consultant for you
You can have a look to the list of all library kits available and the Macrogen Europe sample criteria. RTR samples? You can also find the library criteria in case your sample are ready for sequencing.
For most researches obtaining RAW data is sufficient for their research. However, Macrogen Europe is ready to provide bioinformatics analysis for your project.
Have a look at the top-selling basic and advanced bioinformatics reports for Transcriptome Sequencing
To compare control samples with your sample of the study. Our analysis can provide you with the number of up and down-regulated genes based on fold change and p-value of comparison pair.
To show the distribution of normalized value of each group for comparison pair.
Heatmap shows results of hierarchical clustering analysis (Euclidean Method, Complete Linkage which clusters the similarity of genes and samples by expression level (normalized value) from significant list.
