EZ-seq is a DNA sequencing service developed by Macrogen Europe. It uses 2D Bar-code labels for simple and secure tracking of the samples. It provides DNA sequence data with high reliability with fast turn-around time at reasonable price.
When you make an order, we ship the EZ-seq labels to you via regular mail. All you need to do is to mix the template with sequencing primer you want to use, and ship the samples to our laboratory with EZ-seq labels on the samples.
By the time you make an order, we already have your email address. So the EZ-seq labels issued are automatially linked to your email address, and any sequence data came from the sample with the labels will be directed to your email address. This means that you do not need to log in or fill the complicated order form everytime you have something to sequence.
Introduction about EZ-seq System
Have you ever felt bored of making long lines of order sheet with template and matching primer?
Have you ever been annoyed with so many invoices that came to you every time you send sequencing samples?
Have you ever wondered how much reasonably it was charged for your sequencing service?
Have you ever wanted to have your DNA sequences back earlier?
Have you ever felt it annoying to log in the web site every time you check out the results?
If you have any of above experiences, it is worth giving a try to EZ-seq, because EZ-seq is designed to get rid of such inconveniences. We are quite sure that you will be surprised to see how EZ the DNA sequencing can be.
EZ-seq service has two options, Tube vs 96-well plate, and Purification vs purified template. By combination of these four options, four different services are available. EZ-seq Purification service will go through PCR purification prior to sequencing.
We tried to reduce the amount of information required, but found that there are some essential information without which we cannot properly deliver our service. So we beg your kind understanding for these. Once you've set up the account in our on-line system, you won't need to fill in those information again. Your information shall be encrypted and securely stored in our server, and shall not be used for any other purpose than correct delivery of our service.
In DNA sequencing service, Email address is just like delivery address of the final product. Let's say you orders one vial of Taq polymerase. The most important information in the order will be where the product should be delivered. So, having a proper and secure email address connected to the EZ-seq label is very much important.
Whoever uses the labels, and wherever the samples with labels come from, the sequence data will be directed to the designated email address, so you will need to forward the results to your colleagues. For change of the recipient email address, please refer to the article right below.
Change of delivery email address is the change of final beneficiary, and it is more or less like change of the contract, so it is our recommendation not to do this frequently. But we are quite a flexible in fitting ourselves to your needs, and we can do that for you. But we will need to get the written confirmation from both of two parties. Please contact us if you wish to proceed with this.
No. You can make order from this web page, and there's no need for typing-in or importing the individual reaction information. You know better about your samples than we do, and we do not need to know what you are sequencing. All we do is to do our best with what we got from you!
EZ-seq is 24-hour service. From the moment of sample arrival, it will take between 12 hour ~ 24 hour depending on what time point the samples arrive. But we basically guarantee 24 hours if there's no problem with the sample condition.
We provide the following files.
AB1 : Raw chromatogram file with quality score
SEQ : Sequence in plain text
PHD : Quality score file
PDF : AB1 files in PDF format for image processing or printing
They are compressed in ZIP file and the download link is sent in the result email.
It depends on the sample condition, but in most ideal condition we read about 1,050 bases with Q20 or higher in more than 650 bases.
Macrogen Europe will issue an invoice via email after data delivery. Invoices can be only paid by Credit Card (only VISA and Master) and wire transfer.
(*Ez-seq, Eco-seq and prepayment invoices are issued within 1~3 business days after the order placement)
Beneficiary Name: Macrogen Europe BV
IBAN: NL36 INGB 0008 8449 96
SWIFT Code: INGBNL2A
(* Please be sure to state the invoice number(s) in the description if you pay by wire transfer. Otherwise, your payment would take long to match our record.)
This should be discussed case by case since our billing procedures differ in order categories. Please directly contact us via info@macrogen-europe.com.
Please contact our sales or invoicing department via info@macrogen-europe.com or invoice@macrogen-europe.com.
Macrogen Europe is applying to EU regulation such as Tax and Corporate law. Based on 1968 Sales Tax Act, ar. 12. 3 Wet OB 1968., we are obligated to charge our local VAT:
If you want to confirm the receipt of your payment or if you have any inquiries/issues regarding the payment you made, please send an email directly to ar.eu@macrogen-europe.com.
Our R&D team successfully developed Macrogen's unique technology called MSE based on partial dephosphorylation, and could sepcifically eliminates the molecules with minority, and EZ-seq Purification adopts this technology. So please feel free to mix the primer.
When you make order, you can tell use your preferred primer. We will send this primer along with your labels.
The success rate of sequencing reaction is in proportional to the number of molecules, not the amount of DNA. So even when we have same amout (ng or ug) of DNA, the number of molecules will be highly variable depending on the size of the DNA, that is, the smaller, the better. So generally the success rate of large molecules like BAC of gDNA is much lower than PCR products of plasmid. But by increasing the concentration of template DNA, we can match up the number of molecules to a certain degree. When you want to sequence these large molecules, we advise you to use the DNA with highest concentration possible.
In all cases, the volume of template and primer is 5ul + 5ul to make total of 10ul. The concentration varies depending on the services.
EZ-seq (without purification)
Template : 50~100ng / ul
Primer : 5pmole / ul
EZ-seq Purification
PCR product : 50ng/ul
Primer : 10pmole / ul
Don’t worry. Your usage of label is kept in our LIMS(Laboratory Management Information System) server. Let us know of your situation and we will reissue the labels and send them to you. These labels will have same serial number with your previous labels, so it is important to discard the old labels even though you find them later as our LIMS validates every label by the time it is scanned.
There are three possibilities.
As many people know, the largest portion of sequencing cost is the dye terminator, machinery depreciation, and labor cost. Among these, cost saving in dye terminator already reached its saturation point, so the price comes from the other two. Thanks to the early adoption of our high-throughput sequencers, we already finished depreciation of the machines. Also by automation in the laboratory, the labor cost got much lowered. More than anything else, we are a scientists-dedicated company, and we keep our price to the level that we think is reasonable regardless of the market price.
The door of Macrogen is always open. If you have passion to become a global player in genomics field, please send your introduction with CV to us. Also, many internship possibilities are available. Please feel free to contact us.
Download our NGS service brochure and check out our NGS Platform Suggestion page.
Check out our Blog/News page, or contact us via ngs@macrogen-europe.com
Please request to Macrogen’s sales representatives for a demo report of your chosen application.
Yes, please check out our Privacy Policy and Data Security Policy.
Check out our NGS Sample Submission Guide page.
For more info, please check out our NGS Sample Submission Guide page.
Contact your Macrogen representative to get access to our online shipment system. The following should be included in the package.
Print the 3x shipping labels and 3x commercial invoices, sign and enclose with your shipment and contact your local office of the chosen courier to schedule a pick-up and dispatch for the same day.
Your sample and data will be discarded after 3 months of data delivery. You may extend the period with a special request.
Start by checking the requested input amount from our QC criteria.
DNA:
Include a brief description of extraction and/or enrichment protocol used in the “Comments” fields of the order sheet.
Usually EB (Elution Buffer), DW (Distilled Water), TE (Tris-EDTA) are used, but if the buffer you are using is from a commercial kit, it is likely that is neutral PH hence would not affect further wet-lab procedures.
Certain carriers and pellet paint types are not compatible with aspects of our sequencing technology.
Start by checking the requested input amount from our QC criteria.
RNA:
Include a brief description of extraction and/or enrichment protocol used in the “Comments” fields of the order sheet.
Usually RNase-free water and DEPC-treated water are used, if the buffer you are using is from a commercial kit, it is likely that is neutral PH hence would not affect to further wet lab procedures.
If you are using RNA transport liquid or ethanol, please clearly state the buffer on our order sheet.
Non-robust results have been observed from samples where Lithium Chloride (LiCl), fluorescent pellet paint, or tRNA was used prior to precipitation and resuspension for sample preparation input.
Place sealed individual 1.5ml microcentrifuge tubes in a 50ml screw cap tube for additional insulation during shipment. To prevent sample tubes from moving during shipment, pack any remaining space in the 50ml tube with clean tissue paper prior to sealing. Refer to our packing guideline.
Contact your Macrogen representative to get access to our online shipment system. The following should be included in the package.
Download DNA QC Criteria
Download RNA QC Criteria
If you have prepared and are going to submit a ready-to-run library for sequencing, please meet 5~10nM concentration and >15ul volume criteria.
Download DNA Library QC Criteria
Download RNA Library QC Criteria
Quantity of DNA
Macrogen quantifies the starting genomic material by a fluorescence-based quantification*, rather than a UV-spectrometer-based method.
This is because fluorescence-based methods, which employ a double-stranded DNA specific dye, will specifically and accurately quantitate dsDNA even in the presence of many common contaminants. UV spectrometer methods based on 260 OD readings are prone to overestimating the DNA concentration due to the presence of RNA and other contaminants commonly found in gDNA preparations.
* Picogreen (Invitrogen, cat.#P7589)
Purity of DNA
Absorbance measurements at 260 nm are commonly used to quantify DNA.
The ratio of absorbance at 260nm to absorbance at 280nm is used as an indication of sample purity, and values of 1.8-2.0 are considered indicative of relatively pure DNA.
Condition of DNA
Gel electrophoresis condition: 1% agarose gel, 30min running at 160V, 0.5ul of DNA loaded, with 1kb marker
Size checking of DNA [Upon request, Extra fee will be charged]
Macrogen quantifies RNA samples by a fluorescence-based quantification. We also check their integrity with an RNA Integrity Number (RIN) value using an Agilent Technologies 2100 Bioanalyzer or equivalent.
RNA that has DNA contamination will result in an underestimation of the amount of RNA used. We recommend including a DNase step with the RNA isolation method. However, contaminant DNA would be removed during mRNA purification.
It is very important to use high-quality RNA as the starting material. Use of degraded RNA can result in low yield, over-representation of the 5' ends of the RNA molecules, or failure of the protocol.
To verify the size of final library fragments, we check the template size distribution by running on an Agilent Technologies 2100 Bioanalyzer using a DNA 1000 chip.
In order to achieve the highest quality of data on Illumina sequencing platforms, it is important to create optimum cluster densities across every lane of every flow cell. This requires accurate quantitation of DNA library templates. So, we quantify prepared libraries using qPCR according to the Illumina qPCR quantification protocol guide.
You can use the MD5 checksum to verify the integrity of downloaded files. When you download your result files from the secure FTP links, do not decompress them straight away, but check the md5sum values of the files first and compare them with the provided md5sum in the report. If the values of md5sum are correct, this means that there is no forgery, modification or omission.
Macrogen will issue an invoice after data delivery and payment term is NET 30 days. If you have any questions, please contact your Macrogen sales representative or ngs@macrogen-europe.com.
Wire transfer to the bank account of Macrogen Europe B.V.
Beneficiary: Macrogen Europe B.V.
IBAN: NL36 INGB 0008 8449 96
Bank Info: ING bank
BIC: INGBNL2A
Please contact our invoicing department via invoice@macrogen-europe.com.
If you want to confirm receipt of your payment, or if you have any inquiries/issues regarding a payment you made, please email ar.eu@macrogen-europe.com.
This terms and conditions are applicable to NGS orders placed from March 2015 onwards.
A. Sequencing raw data and its analyzed result will be discarded 3 months after the data delivery. A specific request for long term storage is to be negotiated separately.
B. Whilst we are willing to examine client queries surrounding the data, we dictate that any formal objections should be addressed within 3 months after the data delivery.
C. Processed sample and any unused sample (back-up, QC failed one, etc.) will be discarded 3 months after the data delivery, unless otherwise special request is made.
D. At request, the samples can be returned at the recipient’s cost. Request should be made no later than 1 month after the data delivery. Usual shipping charge is $100 to $200 depending on the region. For the project amount of total $15,000 or above, the shipping and handling charge can be waived.
E. During data storage period, analysis result of your sample might be used for sample quality check process, data validation, internal education or other investigation for better service by Macrogen.
