Now, if you like what you read or you have any extra questions, you can book a free online meeting to discuss your project with our consultants. Tell us about your project and we will get the correct consultant for you.
With Standard-Seq, all your bases are covered. Whether you are sending gDNA, plasmids, purified or non-purified PCR products, there is always a good option for you with our Standard-Seq service.
Create an account on our ordering platform and submit orders right from your lab chair.
Now, if you like what you read or you have any extra questions, you can book a free online meeting to discuss your project with our consultants. Tell us about your project and we will get the correct consultant for you.
With Sanger sequencing technology, we can cover up to 1200 bp of your samples, starting either from purified or unpurified PCR products, or plasmids. If needed, we can also perform gDNA extraction to fit your project.
If you want to be sure your hard work of obtaining your precious samples is rewarded with excellent sequencing results, this is the service for you.
At our facilities, our experienced technical team will premix either single tubes or full plates and sequence your samples on the 3730xl DNA Analyzer. After getting your results, our quality control team will check them to make sure you only receive the highest quality sequencing results.
This is a non-barcoded sequencing service which is useful for one-off projects or if the bulk of your sequencing needs to be done at once. This sequencing comes in the form of individual tubes and 96-well plates. Contact us for more information.
Now, if you like what you read or you have any extra questions, you can book a free online meeting to discuss your project with our consultants. Tell us about your project and we will get the correct consultant for you.
This is a non-barcoded sequencing service which is useful for one-off projects or if the bulk of your sequencing needs to be done at once. This sequencing comes in the form of individual tubes and 96-well plates.
The simplicity of Sanger sequencing is what makes it so fast and cost-effective. The technology uses already established lab protocols such as PCR and gel electrophoresis along with some critical additions to sequence the DNA fragments. Sanger sequencing is also known as the chain-termination method which describes the chain-terminating nucleotides that are added during the PCR step. These are known as ddNTPs and are added in the PCR reaction mix along with dNTPs so that the fragments still elongate as usual. The ddNTPs are vital for this method of sequencing as they stop the elongation step of the PCR reaction, resulting in DNA fragments of different sizes.
The DNA fragments are then separated on a gel which results in a laddered appearance because of their varying sizes. The ddNTPs have four differently coloured fluorophores attached which, when scanned by a laser, signals the type of base that it is bound to. Once the series of bases has been established, you can build your sequence together and now know the complete sequence of your fragment.
Now, if you like what you read or you have any extra questions, you can book a free online meeting to discuss your project with our consultants. Tell us about your project and we will get the correct consultant for you